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Functional complementation of mutant genes of PcINO1 and the hybrid derivative of OsINO1 in S. cerevisiae FY250, an inositol auxotroph. A, Growth of FY250-transformed cells with empty vector, p426GPD (1, −control), ΔPcINO1.1 (2), Os∷PcINO1 (3), ΔPcINO1.2 (4), and PcINO1 (5, +control) on complete synthetic solid media in presence of 10 μm inositol in absence of uracil (ura). B, Growth of transformed cells as in A above in absence of both inositol and uracil (ura).
