Figure 7.

Light scattering property of mutant proteins. Aggregation assay of ΔPcINO1.2 (A) and Os∷PcINO1 (B) in different concentration of NaCl. Approximately 300 μg/mL of each purified protein in 20 mm Tris-HCl (pH 7.5) and 10 mm β-ME was subjected to static light scattering at 360 nm in absence and presence of increasing concentrations of NaCl (100–500 mm) at 37°C in SHIMADZU UV-160A for up to 170 min. Absorbance values at 360 nm were plotted at 10 min intervals as a function of time. Line 1, 0 mm NaCl; line 2, +100 mm NaCl; line 3, +200 mm NaCl; line 4, +300 mm NaCl; line 5, +400 mm NaCl; and line 6, +500 mm NaCl.