Figure 5.

Overexpression of LlANK in lily pollen. A, Diagrammatic representation (not to scale) of both GFP and LlANK-GFP constructs. Zm13, Pollen-specific strong promoter. B, Control GFP evenly distributed in pollen tube. C, Moderately expressed LlANK-GFP associated with granular structures. D, Increasing amount of LlANK-GFP DNA led to abnormal pollen tube growth. Bar = sd. Data were from three independent bombardments. Morphological abnormalities included budding (E), membrane invagination (F), tip ballooning (G), and tube swelling (H). In E to H, green fluorescence (left) and their corresponding transmitted images (right) are juxtaposed. Bar = 10 μm.