Skip to main content
. 2006 Apr;140(4):1306–1316. doi: 10.1104/pp.105.075382

Figure 4.

Figure 4.

The original brx allele is a loss-of-function allele. A, RT-PCR of BRX mRNA, amplified from total RNA isolated from wild-type (Col) or brxC mutant seedlings. −RT, Control reaction without reverse transcriptase. B, Schematic presentation of BRX full-length and potential truncated mutant protein. Light-gray boxes indicate the position of conserved N-terminal domains in BRX (see Fig. 1B) and dark-gray boxes indicate the position of the conserved BRX domains (see Fig. 1, C and D). Respective amino acid positions are indicated. C to D, Relative primary root length of 9-d-old seedlings grown in tissue culture with respect to wild-type control (Sav-0). C, Values for independent transgenic lines expressing the 35S:BRXN140 transgene in brxS background. D, Values for independent transgenic lines expressing the 35S:BRXN140 transgene in Sav-0 background. Error bars indicate se of the mean. Asterisks indicate P values of Student's t test, with one asterisk signifying P < 0.05 and two asterisks signifying P < 0.01.