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. 2006 Apr;140(4):1451–1463. doi: 10.1104/pp.105.070474

Figure 6.

Figure 6.

Effect of water-soluble apoplastic metabolites (AWF filtrate) or water on the consumption of NADH by purified apoplastic proteins. Oxidation of NADH is displayed as changes in absorption at λ = 340 nm per min. AWF from controls and Mn treatments (50 μm for 4 d) from cowpea cv TVu 91 and cv TVu 1987 were divided into two fractions (purified proteins and AWF filtrate) using centrifugal concentrators with MWCO 5 kD. Protein concentrates (A, TVu 91 control; B, TVu 91 Mn; C, TVu 1987 control; and D, TVu 1987 Mn), and AWF-filtrates (a, TVu 91 control; b, TVu 91 Mn; c, TVu 1987 control; and d, TVu 1987 Mn) or water (e) were combined. For measurement of enzyme activities, 1 μL of protein concentrate and 50 μL AWF filtrate or double demineralized water were mixed with 16 mm MnCl2, 1.6 mm p-coumaric acid, and 0.3 mm NADH.