TABLE 1.

Primer specificity for amplification of gene fragments from L. monocytogenes compared to other species of the genus Listeria

Gene target	Annealing temp (°C)	Strain	Amplicon size (bp)	Fluorescencea	Gelb
hly	58	L. monocytogenes ATCC 19115	273	115	+
		L. innocua ATCC 33091		28	−
		L. ivanovii ATCC 19119		27	−
		L. grayi ATCC 25401		30	−
		L. seeligeri ATCC 35967		32
		L. welshimeri ATCC 35897		30	−
		Water (negative control)		26	−
iap	60	L. monocytogenes ATCC 19115	330	189	+
		L. innocua ATCC 33091		45	−
		L. ivanovii ATCC 19119		43	−
		L. grayi ATCC 25401		44	−
		L. seeligeri ATCC 35967		43	−
		L. welshimeri ATCC 35897		46	−
		Water (negative control)		40	−

^aThe values are fluorescence values (excitation wavelength, 485 nm; emission wavelength. 530 nm) obtained with a CytoFluor II plate reader.

^b+ or − indicates the presence or absence, respectively, of bands of the expected size as determined by agarose gel electrophoresis and EtBr staining.