Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Mar 23;7:13. doi: 10.1186/1471-2199-7-13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006 Speek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Tissue-specificity of GLI2 mRNA and its alternatively spliced forms. Southern blot analysis of the PCR products amplified from cDNAs derived from (A) normalized multiple tissue panels and (B) different cell lines (indicated on top of the panel) using GLI2-specific primers derived from exons 2 and 7. The products were hybridized with a 918 nt-riboprobe encompassing GLI2 exons 2–7. Bars on the right indicate sizes of PCR products corresponding to GLI2 mRNA and its alternatively spliced forms GLI2Δ3 and GLI2Δ4–5 (0.9, 0.8 and 0.5 kb, respectively). A shorter and longer exposure were used to determine the presence of minor transcripts corresponding to GLI2Δ3 (0.8 kb) in ovary and testis (panel A). Note the complex pattern of alternatively spliced products observed for cell lines (panel B) that is most likely due to promiscuous splicing (not analyzed in detail). Control, no cDNA.