TABLE 2.
mRNA expression levels in S. cerevisiae strains TMB 3399 and TMB 3400 for genes coding for transporters, enzymes involved in xylose metabolism, the pentose phosphate pathway, glycolysis, glyoxylate shunt, gluconeogenesis, and galactose metabolism
| Type and open reading frame | Gene | Description | Mean mRNA levels (arbitrary units) ± SDa
|
||||
|---|---|---|---|---|---|---|---|
| Glucose
|
Glucose + xylose
|
Xylose (TMB 3400) | |||||
| TMB 3399 | TMB 3400 | TMB 3399 | TMB 3400 | ||||
| Hexose transporters | |||||||
| YHR092C | HXT4 | High-affinity glucose transporter | 37 ± 11 | 25 ± 3 | 139 ± 104 | 55 ± 2 | 8 ± 1 |
| YHR096C | HXT5 | Hexose transporter | 634 ± 75 | 1,038 ± 176 | 654 ± 145 | 883 ± 157 | 1,314 ± 218 |
| YDR342C | HXT7 | Hexose transporter | 1,967 ± 211 | 2,097 ± 124 | 2,476 ± 290 | 1,981 ± 249 | 1,327 ± 36 |
| YLR081W | GAL2 | Galactose permease | 11 ± 3 | 6 ± 2 | 8 ± 5 | 8 ± 7 | 688 ± 19 |
| Xylose metabolism | |||||||
| YHR104W | GRE3 | Aldo-keto-reductase | 160 ± 12 | 209 ± 18 | 209 ± 50 | 249 ± 14 | 306 ± 68 |
| YLR070C | Strong similarity to sugar dehydrogenases | 55 ± 8 | 47 ± 5 | 60 ± 7 | 58 ± 12 | 78 ± 3 | |
| YGR194C | XKS1 | Xylulokinase | 613 ± 87 | 1,278 ± 102 | 773 ± 160 | 1,207 ± 23 | 1,605 ± 176 |
| Pentose phosphate pathway | |||||||
| YNL241C | ZWF1 | Glucose-6-phosphate dehydrogenase | 480 ± 69 | 400 ± 55 | 458 ± 69 | 471 ± 14 | 359 ± 32 |
| YHR163W | SOL3 | Shows similarity to glucose-6-phosphate dehydrogenase non-catalytic domains | 930 ± 169 | 1,577 ± 168 | 932 ± 95 | 1,824 ± 32 | 1,739 ± 168 |
| YHR183W | GND1 | Phosphogluconate dehydrogenase (decarboxylating) | 1,664 ± 210 | 2,383 ± 104 | 1,804 ± 101 | 2,371 ± 114 | 2,396 ± 107 |
| YOR095C | RKI1 | Ribose-5-phosphate ketol-isomerase | 130 ± 4 | 207 ± 38 | 124 ± 18 | 129 ± 7 | 159 ± 27 |
| YJL121C | RPE1 | D-Ribulose-5-phosphate 3-epimerase | 739 ± 13 | 707 ± 35 | 757 ± 181 | 683 ± 57 | 342 ± 38 |
| YLR354C | TAL1 | Transaldolase | 885 ± 66 | 1,124 ± 72 | 1,018 ± 129 | 1,209 ± 28 | 1,213 ± 13 |
| YPR074C | TKL1 | Transketolase 1 | 1,191 ± 34 | 1,595 ± 28 | 1,400 ± 63 | 1,646 ± 209 | 1,428 ± 67 |
| Glycolysis | |||||||
| YFR053C | HXK1 | Hexokinase I (PI) (also called hexokinase A) | 1,250 ± 36 | 1,299 ± 261 | 1,440 ± 40 | 1,267 ± 194 | 700 ± 8 |
| YGL253W | HXK2 | Hexokinase II (PII) (also called hexokinase B) | 890 ± 36 | 782 ± 69 | 873 ± 149 | 877 ± 194 | 317 ± 23 |
| YCL040W | GLK1 | Glucokinase | 1,600 ± 100 | 1,771 ± 317 | 2,286 ± 249 | 1,648 ± 36 | 2,019 ± 125 |
| YDR516C | Strong similarity to glucokinase | 662 ± 44 | 629 ± 69 | 735 ± 32 | 785 ± 44 | 474 ± 100 | |
| YBR196C | PG1 | Glucose-6-phosphate isomerase | 1,349 ± 65 | 1,538 ± 114 | 1,640 ± 26 | 1,427 ± 97 | 1,400 ± 25 |
| YGR240C | PFK1 | Phosphofructokinase alpha subunit | 1,078 ± 56 | 1,157 ± 170 | 1,217 ± 89 | 1,085 ± 51 | 1,254 ± 49 |
| YKL060C | FBA1 | Aldolase | 3,244 ± 580 | 3,830 ± 206 | 4,123 ± 31 | 3,561 ± 550 | 4,194 ± 225 |
| YDR050C | TPI1 | Triosephosphate isomerase | 2,743 ± 483 | 3,196 ± 184 | 3,324 ± 243 | 3,370 ± 426 | 3,452 ± 21 |
| YJL052W | TDH1 | Glyceraldehyde-3-phosphate dehydrogenase 1 | 2,924 ± 600 | 3,156 ± 74 | 3,288 ± 30 | 3,056 ± 516 | 3,397 ± 255 |
| YJR009C | TDH2 | Glyceraldehyde-3-phosphate dehydrogenase 2 | 3,151 ± 466 | 3,856 ± 76 | 4,128 ± 11 | 3,193 ± 623 | 3,861 ± 115 |
| YGR192C | TDH3 | Glyceraldehyde-3-phosphate dehydrogenase 3 | 4,058 ± 1066 | 4,591 ± 250 | 4,451 ± 45 | 4,571 ± 749 | 4,628 ± 553 |
| YCR012W | PGK1 | 3-Phosphoglycerate kinase | 2,983 ± 507 | 3,573 ± 123 | 3,616 ± 58 | 3,560 ± 507 | 3,852 ± 217 |
| YKL152C | GPM1 | Phosphoglycerate mutase | 3,206 ± 582 | 3,371 ± 332 | 3,796 ± 197 | 3,248 ± 429 | 4,037 ± 220 |
| YGR254W | ENO1 | Enolase I | 4,015 ± 656 | 4,410 ± 107 | 4,498 ± 21 | 4,278 ± 373 | 4,152 ± 242 |
| YHR174W | ENO2 | Enolase II | 2,902 ± 191 | 3,402 ± 102 | 3,441 ± 264 | 2,995 ± 369 | 2,527 ± 71 |
| YAL038W | PYK1 | Pyruvate kinase 1 | 3,051 ± 379 | 3,235 ± 35 | 3,617 ± 39 | 3,307 ± 384 | 3,479 ± 187 |
| Gluconeogenesis/glyoxylate shunt | |||||||
| YKR097W | PCK1 | Phosphoenolpyruvate carboxykinase | 381 ± 124 | 239 ± 94 | 252 ± 26 | 193 ± 77 | 694 ± 9 |
| YER065C | ICL1 | Isocitrate lyase | 454 ± 25 | 396 ± 54 | 473 ± 91 | 365 ± 140 | 1,199 ± 62 |
| Galactose metabolism | |||||||
| YBR020W | GAL1 | Galactokinase | 6 ± 0 | 3 ± 0 | 4 ± 2 | 4 ± 1 | 160 ± 22 |
| YBR018C | GAL7 | Galactose-1-phosphate uridyl transferase | 8 ± 2 | 5 ± 0 | 9 ± 1 | 8 ± 1 | 477 ± 2 |
| YBR019C | GAL10 | UDP-glucose 4-epimerase | 11 ± 1 | 10 ± 1 | 14 ± 1 | 13 ± 0 | 333 ± 49 |
a
Values are means of two independent fermentation experiments. High mRNA expression levels in TMB 3400 are indicated in boldface.