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. 2006 Apr 13;34(7):1959–1973. doi: 10.1093/nar/gkl074

Figure 8.

Figure 8

Effect of increasing E.coli RF1, RF2 and RF2-T246S and human eRF1 expression on termination efficiency. Termination efficiency of the prokaryotic 5′Ra UGA Ra3′ (A) and 5′Ab UGA Ra3′ (B) sequence contexts were measured with the 3A′ protein synthesis termination assay (26). Constructs were assayed in the specific E.coli UGA suppressor strain CDJ64. The mean values from three experiments are presented. Error bars are ±SEM. Termination efficiency of the eukaryotic 5′Ra UGA Ra3′ (C) and 5′Ra UGA Ab3′ (D) sequence contexts were measured with the dual luciferase protein synthesis termination assay in vitro. The assay was supplemented with 250 ng and 500 ng of eRF1 where indicated. Readthrough (%) was calculated by comparison to a control construct (UGG). Experiments were performed in triplicate with a single construct of each signal context. Mean values from three experiments are presented. Error bars are ±SEM.