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. 2006 Apr 14;2(4):e32. doi: 10.1371/journal.ppat.0020032

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© 2006 Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) PrP^Sc cleavage occurs after short contact times with Mte surfaces.

(B) The molecular mass protein C2 of the 20S proteasome subunit from BH was unaltered following desorption from Mte.

(C) Cleavage of PrP^Sc present in infected BH was apparent after desorption from Mte.

(D) PrP^Sc desorbed from Mte lost immunoreactivity against an antibody recognizing the N-terminal portion of the mature protein.

(E) PrP^Sc pretreated with PK bound to Mte and did not exhibit further reduction in molecular mass when desorbed.

Immunoblots (A, B, and E) used mAb 3F4. Immunoblots (C and D) employed anti-C2 and R20 polyclonal antibodies, respectively. Pel, PrP^Sc associated with pelleted mineral particles; Sup, unbound PrP^Sc in supernatant.