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SDS-PAGE of BDA from B. borstelensis BCS-1 at different stages of purification. The proteins were analyzed by SDS-12% PAGE. Lane M, molecular mass markers; lane 1, crude extract of E. coli BL21; lane 2, soluble fraction of the crude extract after heat treatment for 30 min at 55°C; lane 3, after Resource Q column chromatography; lane 4, after phenyl-Superose column chromatography.
