TABLE 2.
O2 consumption by cell suspensions of Sporomusa sp. strain TmAO3 under H2-CO2 and N2-CO2 headspaces and O2 consumption by cell-free controls, as determined by using the diffusion-limited assay systema
| Assay mixture | Headspace | O2 flux (nmol min−1)b | Time period (min)c | Amt of O2 consumed (μmol)d |
|---|---|---|---|---|
| Buffer only | N2-CO2 | 170 ± 13 | 6.5 ± 0.5 | 1.1 ± 0.1 |
| Buffer + cellse | N2-CO2 | 224 ± 18 | 73.0 ± 2.5 | 16.4 ± 1.4 |
| Buffer + cellse | H2-CO2 | 224 ± 18 | >360f | >80.6 |
a
See Fig. 1.
b
Calculated by determining the product of the O2 partial pressure in the reservoir and the O2 permeability of the Teflon membrane.
c
Time period between the onset of the O2 flux and the color change of the redox indicator. The values are means ± mean deviations for two independent experiments.
d
Calculated by determining the product of the O2 flux and time before the color change occurred.
e
The assay mixtures with cells contained 0.6 mg of protein.
f
Buffer remained reduced during the whole experiment.