TABLE 1.
Effect of initial sterilization treatments of Terschelling dune soil (experiment 2) on culturable microbial community compositiona
| Soil | Genus (closest match) | % of isolates belonging to group
|
|||
|---|---|---|---|---|---|
| Wk 1 | Wk 3 | Wk 5 | Wk 10 | ||
| Untreated dune soil | Bacillus/Paenibacillus | 5 | ND | ND | ND |
| Cellulomonas | 5 | ND | ND | ND | |
| Cytophaga | 5 | ND | ND | ND | |
| Micrococcus | 2 | ND | ND | ND | |
| Pseudomonas | 2 | ND | ND | ND | |
| Stenotrophomonas/Xanthomonas | 4 | ND | ND | ND | |
| Streptomyces | 18 | ND | ND | ND | |
| Unidentified | 59 | ND | ND | ND | |
| Microwaved | Arthrobacter | 14 | |||
| Bacillus/Paenibacillus | 67 | ||||
| Burkholderia | 14 | ||||
| Pseudomonas | 25 | 9 | 14 | ||
| Stenotrophomonas/Xanthomonas | 19 | 61 | 72 | 71 | |
| Unidentified | 19 | 15 | |||
| Autoclaved and inoculated | Bacillus/Paenibacillus | 25 | 38 | ||
| Cytophaga | 45 | 4 | |||
| Pseudomonas | 10 | 15 | 13 | 13 | |
| Stenotrophomonas/Xanthomonas | 13 | 5 | 25 | ||
| Streptomyces | 50 | 38 | 38 | ||
| Unidentified | 32 | 26 | 11 | ||
| Open incubation | Cytophaga | ND | ND | 25 | |
| Pseudomonas | ND | ND | 53 | 8 | |
| Sphingobacterium | ND | ND | 10 | 3 | |
| Stenotrophomonas/Xanthomonas | ND | ND | 8 | ||
| Unidentified | ND | ND | 37 | 28 | |
| Autoclaved and closed incubation | Bacillus/Paenibacillus | ND | ND | 100 | 100 |
a
Based on whole-cell fatty acid analysis (FAME) of morphologically different colony types. ND, not determined.