TABLE 4.
E. coli host | Medium | OmpT-PA expression level (BPU/g of cell dry wt) |
---|---|---|
DH5α | LB | 115 |
M9Y | 190 | |
K5 | LB | 200 |
M9Y | 430 | |
JM109 | LB | 95 |
M9Y | 405 | |
MC4100 | LB | 182 |
M9Y | 324 | |
BL21(DE3) | LB | 80 |
M9Y | 335 | |
M9 | 510b |
The host strains were transformed with plasmid pPAOT (Cm) and cultivated in different media at 28°C PA activity was measured 6 h after induction with 0.5 mM IPTG. M9, M9 minimal medium.
PA activity was measured 10 h after induction with IPTG.