TABLE 1.
Step | Total activity (U)a (103) | Total protein (mg) (103) | Sp act (U mg−1) | Purifi- cation (fold) | Yield (%) |
---|---|---|---|---|---|
Cell extract | 4.6 | 1.3 | 3.6 | 1 | 100 |
Source 30Q | 1.4 | 0.9 | 15 | 4.2 | 31 |
Resource Q | 0.42 | 0.0022 | 189 | 53 | 9 |
One unit of enzymatic activity is defined as the amount of enzyme that produces 1 μmol of pNA min−1.