TABLE 1.
Strain or plasmid | Relevant characteristicsa | Source or reference |
---|---|---|
Strains | ||
L. lactis | ||
MG1363 | 16 | |
MG5267 | 41 | |
NZ3800 | MG5267 pepN::nisRK | This work |
NZ9870 | MG1363 ccpA::ery | 28 |
NZ9000 | MG1363 pepN::nisRK | 20 |
E. coli MC1061 | 6 | |
B. subtilis ATCC 6633 | ATCCb | |
Plasmids | ||
pNZ4123 | Cmr; pNZ8048 derivative containing a functional E. coli pgmU gene | This work |
pNZ4124 | Cmr; pNZ8048 derivative containing a functional B. subtilis glk gene | This work |
pNZ4030 | Eryr Eps+ | 38 |
pNZ4130 | Tetr Eps+ | 3 |
pNZ9573 | Eryr; nonreplicative lactococcal plasmid; pepN::nisRK | 10 |
pNZ8048 | Cmr; inducible expression vector carrying the nisA promoter | 20 |
EPS+, EPS-producing phenotype; Cmr, chloramphenicol resistant; Eryr, erythromycin resistant; Tetr, tetracycline resistant.
ATCC, American Type Culture Collection.