TABLE 2.

Enzyme activities and sugar nucleotide and sugar-phosphate concentrations in Lactococcus lactis subsp. cremoris MG1363 derivatives grown in M17 medium with Glc or Lac as sole carbon source

Strain (plasmid)	Sugar source	Nisin (ng ml−1)	Relevant enzyme activity (mU of protein−1a)	Sugar-phosphates (μM mg of protein−1)
				Glucose-6P	Fructose-6P	Glucose-1P
NZ3800	Glc	0	ND	23	9.7	39
NZ3800	Lac	0	ND	31	44	37
NZ9870	Glc	0	ND	47	68	46
NZ3800(pNZ4123)	Lac	0	222 ± 42b	30	25	35
	Lac	1	24,767 ± 506b	30	33	33
NZ3800(pNZ4124)	Lac	0	352 ± 51c	5.2	74	10
	Lac	1	9,482 ± 93c	13	290	10

^aValues (± standard deviations) are based on at least two independent experiments. ND, not determined.

^bPgm-specific activity.

^cGlk-specific activity.