TABLE 2.
Strain (plasmid) | Sugar source | Nisin (ng ml−1) | Relevant enzyme activity (mU of protein−1a) | Sugar-phosphates (μM mg of protein−1)
|
||
---|---|---|---|---|---|---|
Glucose-6P | Fructose-6P | Glucose-1P | ||||
NZ3800 | Glc | 0 | ND | 23 | 9.7 | 39 |
NZ3800 | Lac | 0 | ND | 31 | 44 | 37 |
NZ9870 | Glc | 0 | ND | 47 | 68 | 46 |
NZ3800(pNZ4123) | Lac | 0 | 222 ± 42b | 30 | 25 | 35 |
Lac | 1 | 24,767 ± 506b | 30 | 33 | 33 | |
NZ3800(pNZ4124) | Lac | 0 | 352 ± 51c | 5.2 | 74 | 10 |
Lac | 1 | 9,482 ± 93c | 13 | 290 | 10 |
Values (± standard deviations) are based on at least two independent experiments. ND, not determined.
Pgm-specific activity.
Glk-specific activity.