FIG. 2.

Phylogenetic 16S rRNA-based tree reflecting the relationships of the newly isolated sulfate-reducing strain EbS7 to selected members of the δ subclass of the class Proteobacteria. Only nearly full-length sequences (>1,300 bases) were used for the calculations. The tree was corrected by considering the different results obtained with various tree reconstruction algorithms. Members of the α, β, and γ subclasses of the Proteobacteria were used as an outgroup. The scale bar indicates an estimated sequence divergence of 10%. Clones Sva0516 and SB-21 were retrieved from arctic sediment (45) and a sulfate-reducing enrichment culture with benzene (37), respectively. Strain PRTOL is a toluene-degrading sulfate-reducing bacterium (6); strain Tol2, another toluene-degrading sulfate-reducing bacterium, belongs to the Desulfobacter-Desulfobacterium cluster (41). Desulfarculus baarsii is the revised name of Desulfovibrio baarsii (55). The genus name of the former Desulfobacterium anilini is no longer valid; the line of descent of this species should be renamed. DGGE, denaturing gradient gel electrophoresis.