TABLE 1.

Characteristics of the newly isolated sulfate-reducing strain EbS7 and comparison of selected properties of this strain to those of the phylogenetically closely related strains NaphS2 and mXyS1

Strain	Cell size (μm)	G + C content of DNA (mol %)	Utilization of electron donors and carbon sourcesd
			Toluene (1 and 2%)	Ethylbenzene (≤10%)	m-Xylene (1 and 2%)	m-Ethyltoluene (1 and 2%)	Naphthalene (1 and 2%)	Benzoate	Phenylacetate (1 and 4 mM)	3-Phenylpropionate (1 and 4 mM)	Formate(10 and 20 mM)e	Acetate (5 and 10 mM)	n-Hexanoate (1 and 4 mM)	Lactate (1 and 5 mM)	Pyruvate (1 and 5 mM)
EbS7	1.5 by 2-2.3	51.8	−	+	−	−	−	−	+	+	+	−	+	+	+
NaphS2a	1.3 by 1.0-1.9	53.2c	−	−	−	−	+	+	+c	+c	−	+	ND	−	+
mXyS1b	0.6-1 by 1-2	49.0	+	−	+	+	−	+	ND	ND	+	+	ND	−	+

^aData from reference 19.

^bData from reference 22.

^cData from A. Galushko (personal communication).

^dThe test concentrations indicated are the concentrations used only in the present study with strain EbS7. The concentrations expressed as percentages are the concentrations in the HMN carrier phase. The following other compounds were tested with strain EbS7 but were not utilized: benzene, o-ethyltoluene, p-ethyltoluene, n-propylbenzene, and ethylcyclohexane (each at concentrations of 1 and 2% in the carrier phase); styrene (0.5 and 2%); 1-phenylethanol (0.5 and 2 mM); acetophenone (0.5 mM); 2-phenylpropionate (1 and 2 mM); 4-phenylbutyrate (1 and 4 mM); 4-phenylpentanoate (0.5, 1, and 2 mM); mandelate (0.5 and 2 mM); phenol (0.5 and 2 mM); propionate (5 and 10 mM); butyrate (3 and 6 mM); hexadecanoate (0.5 and 1 mM); succinate (1 and 5 mM); fumarate (1 and 5 mM); malate (1 and 5 mM); ethanol (1 and 5 mM); 1-propanol (1 and 5 mM); 2-propanol (1 and 5 mM); glucose (5 mM) and fructose (5 mM); and H₂ (mixed with 20% CO₂ in the headspace; medium with 1 mM acetate as the carbon source). +, growth observed; −, no growth observed; ND, not determined.

^eWithout an additional organic carbon source in the medium.