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Chitinase activity of extracellular proteins after separation in SDS-polyacrylamide gels. 4-MU-(GlcNAc)3 was used as a substrate. Lane 1, E. coli DH5αF′/pCHIBT; lane 2, B. thuringiensis serovar kenyae LBIT-82; lane 3, E. agglomerans ChiA; lane 4, E. coli DH5αF′; lane 5, molecular mass marker (in kilodaltons) (Gibco BRL). The arrow indicates the location of ChiA74.
