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. 2003 Feb;69(2):1295–1298. doi: 10.1128/AEM.69.2.1295-1298.2003

FIG. 3.

FIG. 3.

Time profiles of cell density (OD600 [•]), DCW (grams per liter [▪]), IGF-I fusion protein (grams per liter [○]), and IGF-I fusion protein content in total protein (percent [□]) during the growth of fed-batch cultures of E. coli W3110 harboring pYKM-I1 (A) and E. coli W3110 harboring pYKM-I1 and pACfAZ2 (B). Vertical dashed lines indicate the time of induction with 1 mM IPTG.