. Author manuscript; available in PMC: 2006 Apr 17.

Published in final edited form as: Nat Med. 2005 Jan 30;11(2):183–190. doi: 10.1038/nm1166

Table 1.

Expression profiles of proinflammatory and signaling genes in liver and additional metabolic parameters in wild-type, LIKK and LISR mice.

Encoded protein	HFD/chow (WT)	LIKK/WT (chow)	+/− NaSal (LIKK)	LISR/WT (HFD)
Cytokines & chemokines
IL-1β	3.53 ± 0.92↑^*	3.68 ± 0.95↑^*	0.49 ± 0.11↓^*	1.02 ± 0.24
IL-4	-	1.42 ± 1.03	-	-
IL-6	6.12 ± 0.85↑^*	8.76 ± 1.71↑^**	0.45 ± 0.10↓^**	0.36 ± 0.16↓^**
IL-10	1.43 ± 0.29	1.81 ± 1.07	0.87 ± 0.23	1.71 ± 0.46
IL-11	0.79 ± 0.41	0.40 ± 0.12↓^*	1.17 ± 0.31	0.82 ± 0.13
IL-13	2.58 ± 0.67	0.83 ± 0.13	-	0.64 ± 0.10
IL-15	2.59 ± 0.92	0.96 ± 0.13	-	1.02 ± 0.15
TNF-α	2.58 ± 0.50↑^*	0.60 ± 0.09	0.75 ± 0.15	1.63 ± 0.50
IFN-γ	-	1.39 ± 0.41	-	-
Cx3CL	0.93 ± 0.27	1.05 ± 0.27	-	1.71 ± 0.69
Receptors
CxCL-10	-	1.72 ± 1.02	-	-
IL-1R1	1.80 ± 0.23	2.55 ± 0.62↑^*	0.55 ± 0.12	0.96 ± 0.15
IL-1R2	2.08 ± 0.65	1.21 ± 0.22	0.93 ± 0.27	1.13 ± 0.44
IL-6Rα	0.55 ± 0.09	0.84 ± 0.09	-	-
IL-6ST/gp130	0.75 ± 0.08	1.43 ± 0.13↑^*	0.69 ± 0.20	0.83 ± 0.13
IL-8R	2.61 ± 0.57↑^*	2.67 ± 0.55↑^*	0.66 ± 0.19↓^*	0.38 ± 0.09↓^*
TNFR1a	1.92 ± 0.27↑^*	0.88 ± 0.18	0.77 ± 0.13	0.61 ± 0.09↓^*
IFN-γR	-	1.55 ± 0.27	-	-
TLR-2	3.56 ± 0.85↑^*	5.32 ± 1.14↑^*	0.58 ± 0.14↓^*	0.55 ± 0.05↓^*
Signaling
TLR-4	2.01 ± 0.27↑^*	1.22 ± 0.16	-	0.49 ± 0.11↓^*
SOCS-1	2.21 ± 0.37↑^*	4.50 ± 1.32↑^*	0.49 ± 0.16↓^*	0.52 ± 0.09↓^*
SOCS-2	1.95 ± 0.24↑^*	3.08 ± 0.42↑^*	0.54 ± 0.18↓^*	0.83 ± 0.17
SOCS-3	2.27 ± 0.40↑^*	1.38 ± 0.63↑^*	0.47 ± 0.21↓^*	0.51 ± 0.09↓^*
Stat-3	0.89 ± 0.14	2.94 ± 0.43↑^*	0.89 ± 0.22	0.99 ± 0.07

Metabolic^b
NF-κB activity^a	2.01 ± 0.14↑^**	2.31 ± 0.03↑^**	0.68 ± 0.08↓^*	0.36 ± 0.04↓^**
HOMA-IR^c	2.56 ± 0.47↑^**	1.64 ± 0.21↑^*	0.47 ± 0.06↓^**	0.42 ± 0.12↓^**
Liver triglycerides	0.90 ± 0.13	0.71 ± 0.45↓^*	0.98 ± 0.17	1.88 ± 0.20↑^*

Serum FFA	1.41 ± 0.05↑^*	0.99 ± 0.09	0.93 ± 0.04	0.96 ± 0.05

Quantitative RT-PCR data represent fold increase over control. WT, wild-type (C57BI/6 or unaffected littermates). HFD, high fat diet. Sodium salicylate (NaSal) was administered in the drinking water.

EMSA measurements of NF-κB activity in liver.

Metabolic parameters, not mRNA expression.

HOMA-IR, homeostasis model assessment-insulin resistance (fasting glucose (mmol/L) × fasting insulin (mU/L)/22.5). Statisical analyses: mean ± s.e.m.

P < 0.05

^**

P < 0.01, n = 4–6.