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. 2006 Mar 9;25(7):1481–1491. doi: 10.1038/sj.emboj.7601042

Figure 3.

Figure 3

(A) A stable higher oligomeric complex containing both ClpC-DWB and MecA was detected by gelfiltration in the presence of ATP. The elution profile of size-exclusion chromatography experiments with ClpC-DWB (10 μM) with or without MecA (10 μM), preincubated with ATP (5 mM) and 0.5 mM ATP in the running buffer, as indicated. The relative position and molecular weights of standard proteins are indicated above the elution profile. The size of the higher oligomeric complex (769±3 kDa) determined by multiangle light scattering is indicated. The fractions of the experiment with ClpC-DWB and MecA in the presence of ATP were analyzed as described in Figure 1 and depicted below the elution profile. (B) The EM (76 000 × enlarged) of negatively stained (0.5% uranyl acetate) particles of ClpC-DWB and MecA (10 μM) preincubated with ATP (2 mM) is depicted. The length of the bar is 50 nm.