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. 2006 Mar 9;25(7):1481–1491. doi: 10.1038/sj.emboj.7601042

Figure 6.

Figure 6

(A) The double hybrid ClpA and ClpP can degrade MecA. The Coomassie-stained SDS–PAGE of a degradation assay with E. coli ClpA or CNTD-ClpA-CLi together with ClpP. (B) MecA can target ComK for degradation by the CNTD-ClpA-CLi and ClpP. The Coomassie stained SDS–PAGE of a degradation assay with B. subtilis ClpCP or E. coli CNTD-ClpA-CLi with ClpP showing the degradation of ComK-MBP in the presence or absence of MecA. All proteins were used at a final concentration of 1 μM. The incubation time is indicated below the lanes.