TABLE 1.
Number | Name | Sequence (5′-3′)a |
---|---|---|
1 | rLuc-IRES(+) | CCGAGCTCAGACTTCGAAAGTTTATGATCC* |
2 | rLuc (−) | GGTCTAGAATTGTTCATTTTTGAGAACTC |
3 | poly(A)12 (+) | CTAG(A)12 |
4 | poly(A)12 (−) | CGA(T)12 |
5 | poly(A)50 (+) | CTAG(A)50 |
6 | poly(A)50 (−) | CGA(T)50 |
7 | CBV3 3′UTR (+) | CCTCTAGATTAGAGACAATTTG |
8 | CBV3+A12 3′UTR (−) | GGATCGA(T)12CCGCACCGAATGC |
9 | CBV3 3′UTR (−) | CCATCGATCCGCACCGAATGCGGAGA |
10 | T7/HRV2 5′UTR (+) | CGGCGGCCGCTAATACGACTCACTATAGGT TAAAACTGGA |
11 | HRV2 5′UTR (−) | CTGAGCTCCCATGGTGCCAATATATATATTG |
12 | HRV2 3′UTR (+) | CTAGATATAGAAATAGTAAACTGATAGTT TATTAGTTTTAT |
13 | HRV2 3′UTR (−) | CTAGATAAAACTAATAAACTATCAGTTT ACTATTTCTATAT |
14 | T7/PV1 5′UTR (+) | CGGGATCCTAATACGACTCACTATAGGT TAAAACAGCTCTGG |
15 | PV1 5′UTR (−) | CTGAGCTCCCATTATGATACAATTGTCTG |
16 | PV1 3′UTR (+) | GGTCTAGAAACCCTACCTCAGTCG |
17 | PV1 3′UTR (−) | GGGTCGAC(T)50CTCCGAATTA |
18 | CBV3 ORF (+) | GGGAGCTCAAGTATCAACGCAAAAG |
19 | CBV3 1/2 VP4 (−) | CTTTCGAAGTTGCGGCATCCTTGTAATAA TTAAC |
20 | CBV3 VP4 (−) | CTTTCGAAGTGTTGAGAGCTGGTAGTGATT |
21 | CBV3 1/3 VP0 (−) | CTTTCGAAGTGTCTGGTTGGGTCGGTTGG |
22 | HRV2 ORF (+) | GGGAGCTCAGGTTTCAAGACAAATGTTGG |
23 | HRV2 1/2 VP4 (−) | CTTTCGAAGTTGAAGCAGCATCTTTGAAAT |
24 | HRV2 VP4 (−) | CTTTCGAAGTCTGTAGTGTTGGTATTCCC |
25 | HRV2 1/3 VP0 (−) | CTTTCGAAGTGTTTTCACCAAAAATACCC |
26 | PV1 ORF (+) | GGGAGCTCAGGTTTCATCACAGAA |
27 | PV1 1/2 VP4 (−) | GTTTCGAAGCCGCGTTACTAGC |
28 | PV1 VP4 (−) | CTTTCGAAGTGTTTAGCATTGGGGCTGTT |
29 | PV1 1/3 VP0 (−) | CTTTCGAAGTTTGCCCAAAGAGTCCCATG |
30 | PV1-IRES (+) | CAACGCGTAAAACCAAGTTCAATAGAAGG |
31 | PV1-IRES (−) | CTGAGCTCCCATTATGATACAATTGTCTG |
32 | PV1 5′ junction (+) | CCAAGGTCACCTCCAAAATCAG |
33 | PV1 5′ junction (−) | CAAATGCGCCATATGTGGTCAGATCCTTGG |
34 | CBV3-2A (+) | GACCACATATGGCGCATTTGGACAACAATC |
35 | CBV3-2A (−) | CAATGGAACAGGGCATCACCAATTACA TAGAG |
36 | PV1 3′ junction (+) | TGGTGATGCCCTGTTCCATTGCATCATCTTC |
37 | PV1 3′ junction (−) | GGTACTGGTCACCATATTAGTC |
38 | rLuc-TNT(+) | CCCTCGAGACCATGACTTCGAAAGTTTATG ATCC |
39 | CBV3 2A(+) | CCCTCGAGACCATGACTTCGAAAGTTTATG ATCC |
40 | CBV3 2A(−) | TCTCTAGAACTGTTCCATTGCATCATCTTCC |
41 | PV1 2A(+) | CACTCGAGACCATGGGATTCGGACACCAAA ACAAAGC |
42 | PV1 2A(−) | CAGGTCGACCTATTGTTCCATGGCTTCTT CTTC |
43 | CBV3 2A Cys-Ala(−) | ACCGCCGGCGTCACCTGGTTCGGAAAATCC |
44 | CBV3 2A Cys-Ala(+) | GGTGACGCCGGCGGTATCCTAAGGTGTG |
45 | c-myc (+) | CTAGCACCATGGAGCAGAAACTCATCTCTG AAGAGGATCTGA |
46 | c-myc (−) | AGCTTCAGATCCTCTTCAGAGATGAGTTT CTGCTCCATGGTG |
47 | eIF4GI-b (+) | CCAAGCTTATGAACACGCCTTCTCAGCC |
48 | eIF4GI-e (+) | TTAAGCTTATGTCTGGGGCCCGCACTG |
49 | eIF4GI (−) | CAGCGGCCGCTCAGTTGTGGTCAGACTCC |
50 | cpN(−) | TTGCGGCCGCTCAACGGGTGCTAAGGGTT GTCC |
51 | cpC(+) | TTAAGCTTGGGCCCCCAAGGGGTGG |
52 | eIF4GI-R (−) | TGGCCCACCCCTTGGAGGCTCACGGGTGCTA AGGGTTGTGCGCGCAAGGTTGGCAAAGG ATGG |
53 | eIF4GI-Sbf (+) | CTCCTGCAGGCAGCAATCCAGGC |
Restriction sites used for cloning are underlined.