TABLE 1.
List of oligonucleotides used in this work
| Number | Name | Sequence (5′-3′)a |
|---|---|---|
| 1 | rLuc-IRES(+) | CCGAGCTCAGACTTCGAAAGTTTATGATCC* |
| 2 | rLuc (−) | GGTCTAGAATTGTTCATTTTTGAGAACTC |
| 3 | poly(A)12 (+) | CTAG(A)12 |
| 4 | poly(A)12 (−) | CGA(T)12 |
| 5 | poly(A)50 (+) | CTAG(A)50 |
| 6 | poly(A)50 (−) | CGA(T)50 |
| 7 | CBV3 3′UTR (+) | CCTCTAGATTAGAGACAATTTG |
| 8 | CBV3+A12 3′UTR (−) | GGATCGA(T)12CCGCACCGAATGC |
| 9 | CBV3 3′UTR (−) | CCATCGATCCGCACCGAATGCGGAGA |
| 10 | T7/HRV2 5′UTR (+) | CGGCGGCCGCTAATACGACTCACTATAGGT TAAAACTGGA |
| 11 | HRV2 5′UTR (−) | CTGAGCTCCCATGGTGCCAATATATATATTG |
| 12 | HRV2 3′UTR (+) | CTAGATATAGAAATAGTAAACTGATAGTT TATTAGTTTTAT |
| 13 | HRV2 3′UTR (−) | CTAGATAAAACTAATAAACTATCAGTTT ACTATTTCTATAT |
| 14 | T7/PV1 5′UTR (+) | CGGGATCCTAATACGACTCACTATAGGT TAAAACAGCTCTGG |
| 15 | PV1 5′UTR (−) | CTGAGCTCCCATTATGATACAATTGTCTG |
| 16 | PV1 3′UTR (+) | GGTCTAGAAACCCTACCTCAGTCG |
| 17 | PV1 3′UTR (−) | GGGTCGAC(T)50CTCCGAATTA |
| 18 | CBV3 ORF (+) | GGGAGCTCAAGTATCAACGCAAAAG |
| 19 | CBV3 1/2 VP4 (−) | CTTTCGAAGTTGCGGCATCCTTGTAATAA TTAAC |
| 20 | CBV3 VP4 (−) | CTTTCGAAGTGTTGAGAGCTGGTAGTGATT |
| 21 | CBV3 1/3 VP0 (−) | CTTTCGAAGTGTCTGGTTGGGTCGGTTGG |
| 22 | HRV2 ORF (+) | GGGAGCTCAGGTTTCAAGACAAATGTTGG |
| 23 | HRV2 1/2 VP4 (−) | CTTTCGAAGTTGAAGCAGCATCTTTGAAAT |
| 24 | HRV2 VP4 (−) | CTTTCGAAGTCTGTAGTGTTGGTATTCCC |
| 25 | HRV2 1/3 VP0 (−) | CTTTCGAAGTGTTTTCACCAAAAATACCC |
| 26 | PV1 ORF (+) | GGGAGCTCAGGTTTCATCACAGAA |
| 27 | PV1 1/2 VP4 (−) | GTTTCGAAGCCGCGTTACTAGC |
| 28 | PV1 VP4 (−) | CTTTCGAAGTGTTTAGCATTGGGGCTGTT |
| 29 | PV1 1/3 VP0 (−) | CTTTCGAAGTTTGCCCAAAGAGTCCCATG |
| 30 | PV1-IRES (+) | CAACGCGTAAAACCAAGTTCAATAGAAGG |
| 31 | PV1-IRES (−) | CTGAGCTCCCATTATGATACAATTGTCTG |
| 32 | PV1 5′ junction (+) | CCAAGGTCACCTCCAAAATCAG |
| 33 | PV1 5′ junction (−) | CAAATGCGCCATATGTGGTCAGATCCTTGG |
| 34 | CBV3-2A (+) | GACCACATATGGCGCATTTGGACAACAATC |
| 35 | CBV3-2A (−) | CAATGGAACAGGGCATCACCAATTACA TAGAG |
| 36 | PV1 3′ junction (+) | TGGTGATGCCCTGTTCCATTGCATCATCTTC |
| 37 | PV1 3′ junction (−) | GGTACTGGTCACCATATTAGTC |
| 38 | rLuc-TNT(+) | CCCTCGAGACCATGACTTCGAAAGTTTATG ATCC |
| 39 | CBV3 2A(+) | CCCTCGAGACCATGACTTCGAAAGTTTATG ATCC |
| 40 | CBV3 2A(−) | TCTCTAGAACTGTTCCATTGCATCATCTTCC |
| 41 | PV1 2A(+) | CACTCGAGACCATGGGATTCGGACACCAAA ACAAAGC |
| 42 | PV1 2A(−) | CAGGTCGACCTATTGTTCCATGGCTTCTT CTTC |
| 43 | CBV3 2A Cys-Ala(−) | ACCGCCGGCGTCACCTGGTTCGGAAAATCC |
| 44 | CBV3 2A Cys-Ala(+) | GGTGACGCCGGCGGTATCCTAAGGTGTG |
| 45 | c-myc (+) | CTAGCACCATGGAGCAGAAACTCATCTCTG AAGAGGATCTGA |
| 46 | c-myc (−) | AGCTTCAGATCCTCTTCAGAGATGAGTTT CTGCTCCATGGTG |
| 47 | eIF4GI-b (+) | CCAAGCTTATGAACACGCCTTCTCAGCC |
| 48 | eIF4GI-e (+) | TTAAGCTTATGTCTGGGGCCCGCACTG |
| 49 | eIF4GI (−) | CAGCGGCCGCTCAGTTGTGGTCAGACTCC |
| 50 | cpN(−) | TTGCGGCCGCTCAACGGGTGCTAAGGGTT GTCC |
| 51 | cpC(+) | TTAAGCTTGGGCCCCCAAGGGGTGG |
| 52 | eIF4GI-R (−) | TGGCCCACCCCTTGGAGGCTCACGGGTGCTA AGGGTTGTGCGCGCAAGGTTGGCAAAGG ATGG |
| 53 | eIF4GI-Sbf (+) | CTCCTGCAGGCAGCAATCCAGGC |
a
Restriction sites used for cloning are underlined.