FIG.4.

Recovery of the accumulation of processed HDV RNA transcripts following ribavirin treatment. HDV genome replication in cultures of 293-HDV cells was induced and assayed as described in the legends for Fig. 1 and 2. For two of three cultures (open circles), the inhibitors ribavirin (rib) and MPA, as indicated, were used at concentrations of 30 μM and 1 μM, respectively. After 24 h, the medium for each of the three cultures was replaced with medium with no TET and containing 50 μg/ml of cycloheximide (cyclo). After an additional 18 h, total RNA was extracted and Northern analysis was used to quantitate the accumulation of unit-length genomic HDV RNA.