FIG. 3.

Detection of RRV genomes in persistently infected B-cell lines by FISH. RRV-infected B-cell lines and corresponding parental cell lines were fixed and hybridized in situ with a SpectrumRed-labeled RRV pooled-cosmid probe that covered 90% of the unique coding region. Cell nuclei were counterstained with DAPI (blue). Results are shown for the RRV-infected 211-98 cell line (A and B) and the parental RRV-negative cell line (C). Staining patterns observed were consistent with latent persistence of RRV (A) and, at a lower frequency, productive replication (B). No RRV-specific staining was observed in the RRV-negative 211-98 parental cell line (C).