FIG. 6.

Neutralization of RRV-GFP infection. RRV-GFP (MOI = 0.3 PFU/cell) was incubated with either medium alone (No Antibody) or sera from RRV-negative rhesus monkeys (Mm 288-94 and 232-03) or rhesus monkeys naturally infected with RRV (Mm 526-91, 488-03, 541-03, 140-83, and 288-03) at a dilution of 1:20 or 1:100. The number of PFU of RRV-GFP was determined on rhesus monkey fibroblasts. After incubating of the virus-serum mixture for 3 h at 37°C with gentle rocking, LCL211-98 cells were inoculated with either medium alone (No Virus) or the virus-serum mixture. At day 4 postinfection, cultures were examined for GFP expression by FACS analysis. The results shown are the percentage of GFP-positive cells along with the standard deviation for each serum dilution tested.