FIG. 5.

Immunoprecipitation-RT-PCR of DRBP76 from HEK-293 cells transfected with PV-RIPO (A) and PV (B) subgenomic RNAs, as well as cells infected with PV-RIPO (C and D). Total RNA or immunoprecipitates generated with α-DRBP76, nonspecific IgG, or α-2C antibodies as indicated were subjected to RT-PCR amplification of the HRV2 (A and C), PV (B), or a c-myc 5′UTR (D). −RT and +RT, reactions carried out in the absence and presence of reverse transcriptase, respectively. (E) Western blot detection of DRBP76 in the immunoprecipitates generated with α-DRBP76 and nonspecific IgG antibodies as indicated.