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. 2006 Apr;80(7):3147–3156. doi: 10.1128/JVI.80.7.3147-3156.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 6. — DRBP76 depletion enhances PV-RIPO propagation in HEK-293 cells. (A) Schematic depiction of shRNA targeting the DRBP76 mRNA. (B) Western blot analysis of control cells (lane 1), shDRBP76 cells (lane 2), and shDRBP76 cells transfected with DRBP76^mut DNA (lane 3) using α-DRBP76 and α-tubulin antibodies as indicated. One-step growth curve analysis of PV-RIPO (C) and PV (E) propagation in control cells (▪), shDRBP76 cells (•), or shDRBP76 cells transfected with pDRBP76^mut DNA (⧫) is shown. Western blot analysis of PV-RIPO (D) and PV (F) proteins in infected cell lysates at specified hours postinfection using α-2C antibody is shown.