FIG. 7.

DRBP76 represses HRV2 IRES-driven reporter translation. (A) Schematic depiction of the HRV2 IRES rLuc reporter expression construct (top) and the capped fLuc reporter construct containing the β-globin 5′UTR (bottom). (B) Effect of DRBP76 knockdown on HRV2 (black columns) or PV (gray columns) IRES-driven rLuc translation relative to capped fLuc translation in shDRBP76 and control HEK-293 cells. The data are the average of three independent assays plus standard error and are expressed as the fold induction of rLuc activity relative to fLuc activity. (C) DRBP76 depletion does not affect the stability of the HRV2 IRES reporter construct in vivo. ³²P-labeled HRV2 IRES reporter RNAs recovered from transfected or untransfected (−) shDRBP76 and control cells were analyzed on a denaturing polyacrylamide gel. (D) Kinetics of labeled HRV2 IRES reporter RNA decay by phosphorimager quantification in control (▪) and shDRBP76 (•) cells.