FIG. 1.

Map of the gE CT domain and truncation mutants. The HSV-1 gE CT domain is 106 residues and C terminal to the 25-amino-acid transmembrane (TM) domain, which ends at amino acid 445. There are three arginine (R) residues next the transmembrane domain; two tyrosine motifs (YXXØ) at positions 463 to 466 and 472 to 475; three serines that are phosphorylated at residues 476, 477, and 503; and a cluster of acidic residues located between residues 476 and 484. Mutations in the CT domain were constructed by inserting two tandem stop codons in place of residue 519, 495, 470, or 448. The numbering of gE includes the signal sequence and is based on the previously published sequence of HSV-1 strain 17 gE (29), whereas HSV-1 strain F gE (used here) contains two additional residues in the ET domain (48). Wt, wild type.