FIG. 3.

Cell-to-cell spread of HSV gE CT domain mutants. Human HaCaT keratinocytes (A) or ARPE-19 retinal epithelial cells (B) were infected with F-BAC, F-BACΔgE, gE-448, gE-470, gE-495, or gE-519 using a low multiplicity of infection (30 to 100 PFU/35-mm dish). After 72 h, cells were stained with anti-HSV polyclonal antibodies and peroxidase-conjugated secondary antibodies. Ten plaques were photographed, and NIH Image software was used to calculate plaque areas. The numbers shown in each panel are the average areas ± standard deviations for each set of plaques compared to F-BAC plaques arbitrarily set at 100.