FIG. 8.

Cartoon summarizing traffic of gE mutants. In cells infected with mutant gE-519 and wild-type HSV-1, gE/gI is initially found in the TGN but the moves to cell junctions, and these viruses spread well. gE/gI expressed in gE-470- and gE-495-infected cells localizes initially to the TGN and then remains primarily in cytosolic vesicles, some underlying the plasma membrane, and these viruses spread poorly. In gE-448-infected cells, gE/gI does not accumulate in the TGN and remains distributed throughout the cytoplasm (cyto) and on apical and cell surfaces, and this mutant spreads poorly. nuc, nucleus.