FIG. 2.

Effect of enzymatic removal of GAG from the cell surface. 3D4 cells were digested with one or a combination of two GAG lyases for 60 min at 37°C. The enzymes were washed off, and cells were inoculated with PCV2 for 60 min at 37°C in a 5% CO₂ atmosphere. Cells were then washed and overlaid with monocyte/macrophage medium. After 36 h, cells were fixed and stained for PCV2 antigens. Points represent the relative percentages of PCV2 antigen positive cells from three experiments ± standard deviations.