FIG. 3.

Infection of PCV2 in CHO cell lines. Semiconfluent monolayers of wild-type CHO-K1 cells and of two CHO mutants, pgsD-667 (lacking HS but not CS) and pgsA-745 (lacking both HS and CS) were inoculated with PCV2 at 37°C for 1 h. Thereafter, the viral inoculum was washed off and cells were further incubated with CHO medium for 36 h. Cells were then fixed and stained, and PCV2-antigen positive cells were counted as described in Materials and Methods. Data represent means ± standard deviations of results from three experiments.