FIG. 2.

Distribution and colocalization of VP5, gG, and UL37 proteins in distal axons and growth cones of HSV-1-infected human DRG at 24 h p.i. Human DRG explants were grown on Matrigel-coated coverslips and infected with vUL37-GFP virus. After 24 h p.i., the cultures were fixed, permeabilized, and labeled simultaneously with antibodies to capsid VP5 and envelope glycoprotein gG. VP5, UL37, and gG antigens were distributed throughout the axon with concentrations of fluorescence at varicosities and growth cones (A, B, C). Foci of colocalization between two or three antigens were found at the varicosities and growth cones (arrows) (D to G). The images show UL37-GFP as green (A), VP5 as red (B), and gG as cyan (C). Overlay images of dual colocalization in yellow and free (not colocalized) antigens in blue are shown for VP5 and UL37 (D), for UL37 and gG (E), and for VP5 and gG (F). Triple colocalization (white) and noncolocalized antigens (blue) are shown in panel G. Bars, 40 μm.