FIG. 4.

VP5, gG, and UL37 proteins colocalized in axonal varicosities and growth cones in distal regions of axons of HSV-1-infected human DRG at 48 h p.i. Cultures of DRG explants were infected with vUL37-GFP virus, fixed, permeabilized, and labeled simultaneously with antibodies to VP5 and gG. All three antigens were present in intense foci in varicosities (arrows) and growth cones (arrowheads) (A, B, C). Foci of colocalization of two or three antigens were found in varicosities (arrows) and growth cones (arrowheads). The images show UL37-GFP as green (A), VP5 as red (B), and gG as cyan (C). Overlay images of dual colocalization in yellow and free (not colocalized) antigens in blue are shown for VP5/UL37 (D), for UL37/gG (E), and for VP5/gG (F). Triple colocalization (white) and free antigen (blue) are shown in panel G. Bars, 40 μm.