FIG. 6.

Electron micrographs of HSV-1 particles in infected human DRG axons. Coverslips with explanted DRG cultures in situ were infected with the clinical isolate CW1 of HSV-1, fixed, and processed for TEM. Areas containing mid- and distal regions of axons were sectioned and examined. (A) Unenveloped capsid (arrowhead) in midaxon. (B) Partially enveloped capsid (arrowhead) in an expanded, vesicle-rich varicosity. The inset shows an enlargement of the viral particle, surrounded by a vesicular membrane. (C) Enveloped capsid in a vesicle (arrow) in a varicosity. The inset shows an enlargement of the enveloped capsid in a vesicle. (D) Extracellular viral particle (arrowhead) adjacent to a varicosity. The inset shows an enlargement of the viral particle in a thickened crescent-shaped invagination of the axonal plasma membrane. Bars, 200 nm.