FIG. 9.

Electron micrographs of HSV-1 particles in infected human DRG axons. Coverslips with explanted DRG cultures in situ were infected with the clinical isolate CW1 of HSV-1, fixed, and processed for electron microscopy. (A) Enveloped (arrow) and unenveloped (arrowhead) viral particles in varicosities. The membrane of the vesicle surrounding the enveloped capsid (inset) has fused with the axonal membrane, forming a pore to the surface, presumably allowing the virion to exit the axon. (B) Immunogold labeling of growth cones for tegument protein VP22 (5 nm) (arrows) and glycoprotein D (10 nm) (arrowheads). Label for VP22 (5 nm) is associated with vesicles either alone or together with gD (10 nm). Bars, 200 nm.