FIG. 3.
Staining of PBMC from 10 HLA-A*0201+ healthy individuals with tetrameric HLA-A*0201/BKV VP1p44 and HLA-A*0201/BKV VP1p108 complexes after in vitro stimulation with the respective peptides for 10 to 14 days. The percentages of CD8αβ+ T cells that bind the tetramers (right upper quadrant of each panel) are indicated in each panel. BKV VP1p44 tetramer-positive cells were detected in 8 of 10 healthy individuals (left column), while BKV VP1p108-positive cells were present in 5 of 10 individuals of the same group (right column). Results were considered positive if the percentage of tetramer-staining cells was equal or greater than 0.1% of CD8αβ+ T cells and formed a distinct population of cells on the dot plot.