FIG. 4.

(A) Antibody response against HTLV-1 from each rabbit was measured by anti-HTLV-1 ELISA, using both HTLV-1 whole-virus lysates and envelope protein as antigens. Each bar represents an average absorbance value for six samples from each group (729.ACH or 729.ACH.p13) ± the standard deviation. (B) Anti-HTLV-1 Western immunoblot. Rabbit sera were tested for antibody response to specific HTLV-1 viral antigens. Representative results from selected animals are shown. Rabbit H5 was inoculated with wild-type 729.ACH cells, whereas H12 and H13 were inoculated with 729.ACH.p13 cells. rgp46, HTLV-1-specific recombinant envelope surface protein; p53, Gag precursor; p24, capsid; p19, matrix; GD21, recombinant envelope transmembrane protein; 729(−), uninfected, HTLV-1-negative cells; *, serum loading control bands indicating comparable concentrations of serum immunoglobulin levels among the samples; **, nonspecific seroreactivity against human cell (i.e., the inoculum) antigen; ID, identification; p.i., postinoculation.