FIG. 6.
(A) HTLV-1 proviral loads in infected rabbit PBMC. Viral loads in the infected rabbit PBMC were determined and compared using HTLV-1 tax-specific quantitative real-time PCR. Average viral copy numbers of six rabbits for each inoculum from preinoculation (week 0) and 2- and 8-week postinoculation samplings are shown. *, below detection sensitivity (81 proviral copies per 106 PBMC). Horizontal bars indicate average copy numbers (n = 6). (B) PCR amplification (445-bp products) of the HTLV-1 provirus from 729.ACH-inoculated rabbit PBMC DNA (H3 to H8) compared to levels of amplification of the virus with the designated mutation site from the rabbit PBMC DNA (H9 to H14) at 8 weeks postinoculation. Rabbits H3 through H8 and H9 through H14 were inoculated with 729.ACH and 729.ACH.p13 cells, respectively. Rabbit H2 was inoculated with the mock control cell line. The plasmids (ACH and ACH.p13) were used as positive controls. MM, PCR master mix.