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. 2006 Apr;80(7):3445–3458. doi: 10.1128/JVI.80.7.3445-3458.2006

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FIG. 2. — RTA reverses LANA-mediated down-regulation of K1p. pGL3B K1p(−650 to +76) was cotransfected into 293 cells with 10 μg of LANA (lane 2) and also with RTA at 5 and 10 μg in lanes 3 and 4, respectively. Increasing amounts of RTA reversed as well as up-regulated K1 promoter activity in a dose-dependent manner. LBS-deleted (−350 to +76) K1p showed synergistic effects of LANA and RTA on K1p transcriptional activity (lanes 3 and 4). Western blots show the expression of LANA and RTA in cotransfected cells. The blot was stripped and reprobed with anti-β-actin antibody to show equal protein loading.