FIG. 6.

Effect of forskolin treatment on the accumulation of phospho-Akt. For lanes 1 to 16, HEp-2 cells were either mock infected or exposed to 10 PFU of HSV-1(F) or ΔUS3 per cell for 1 h. The cells were then exposed to medium containing forskolin (100 μg/ml) initially dissolved in ethanol or medium containing an equivalent amount of ethanol for 2.5 h. For lanes 17 to 24, the cells were maintained in DMEM supplemented with 5% NBCS for 3 h, then treated with forskolin as described above, and harvested at 17 h postinfection. The cells were solubilized, and equal amounts of protein lysates were subjected to electrophoresis on a denaturing gel, and reacted with antibody against PARP or against either total PKB/Akt or PKB/Akt P-473.