FIG. 3.

Effect of HSV infection on specific activity of the Src protein kinase. HEL cells on T75 flasks were mock infected or exposed to 10 PFU of HSV-1(F) per cell. The cells were harvested 0, 3, 6, 9, and 12 h after infection and lysed. Then, Src kinase from each sample was immune precipitated with anti-Src monoclonal antibody and reacted with 10 μg acid-denatured enolase and 10 μCi [γ-³²P]ATP. The phosphorylated enolase was resolved in a 10% polyacrylamide gel, transferred to a nitrocellulose membrane, and scanned/quantified with a General Dynamics Storm 860 phosphorimager as described in Materials and Methods. The total protein level of Src from each sample was also quantified by its reactivity with anti-Src antibody. The determination of the specific activity is described in Results.