FIG. 4.
Effects of HSV infection on the phosphorylation status of Src family kinases in HEL cells. Replicate cultures of HEL fibroblasts in T-25 flasks were mock infected or exposed to 10 PFU of HSV-1(F), ΔICP0, ΔUS3, ΔUL13, or ΔUS3/ΔUL13 mutant viruses per cell. The cells were harvested 4, 8, or 12 h after infection and lysed as described in Materials and Methods, and 120 μg cell lysates from each sample was resolved in a denaturing gel, transferred to a nitrocellulose sheet, and reacted with anti-Src-pY416, anti-Src-pY527, anti-panSrc, anti-ICP0, anti-TK, or anti-actin, respectively.