FIG. 7.

Modification of SFKs in HEL cells infected with HSV-1(F) virus is due to phosphorylation. HEL cells were mock infected or exposed to 10 PFU of HSV-1(F) per cell. At 9 h after infection, the cells were harvested and lysed in RIPA buffer without phosphatase inhibitors. Then, phosphatase inhibitors were added to an aliquot of the cell lysates to serve as input control before phosphatase treatment. The rest of the lysates (400 μg each) were digested with alkaline phosphatase at 30°C for 40 min. One hundred micrograms of total protein was electrophoretically separated in denaturing gels, transferred to a nitrocellulose sheet, and probed with anti-panSrc or anti-Src antibodies.