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. 2006 Apr;80(8):4114–4121. doi: 10.1128/JVI.80.8.4114-4121.2006

FIG. 1.

FIG. 1.

Properties of the eGFP/NS1 plasmid. (A) Plasmid map of eGFP/NS1. The plasmid contains the coding sequence for NS1 fused to the C-terminal end of eGFP, under the control of the ecdysone-inducible response element. Three SP1 enhancers act to increase transcription. This vector is used in tandem with the plasmid pVGRXR, which expresses the heterodimeric ecdysteroid receptor. The inducing agent ponasterone A, a homologue of the insect steroid ecdysone, binds to the ecdysteroid receptor, and the complex activates transcription through binding to the ecdysone-inducible response element. The plasmid is selectable in E. coli with ampicillin and in mammalian cells with Geneticin. BGH, bovine growth hormone. (B) Protein sequence of the NS1 portion of eGFP/NS1 compared to the prototypic sequence (Au) (28) and the sequence of an infectious viral clone (Zhi) (31). Differences between sequences are highlighted in gray.